| 质粒名称: | pET28a-Beclin1 (1-242) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 24401 |
| 存储实验室: | Qing Zhong |
|
载体骨架基本信息 |
|
| 载体名称: | pET-28a |
| 空载体大小: | 5400bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Beclin1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 726 bp |
| 基因突变: | Contains amino acids 1-242 only. |
| 标签/融合蛋白: | His (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (unknown if destroyed) |
| 3’克隆位点: | NotI (unknown if destroyed) |
| 5’测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | pET28a-Beclin1 (1-242) was a gift from Qing Zhong (Addgene plasmid # 24401 ; http://n2t.net/addgene:24401 ; RRID:Addgene_24401) |
| 参考文献部分: | Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase. Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q. Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6. 10.1073/pnas.0810452105 PubMed 19050071 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pcDNA4-Vps34-Flag
- 下一篇：pEGFP-N1-Barkor
